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. 2019 May 6;116(21):10547–10556. doi: 10.1073/pnas.1820810116

Fig. 8.

Fig. 8.

Sperm sncRNA profile in F1 fathers and differences in transcriptomes in brain of F1 father and tsRNA offspring. (A) Length distribution of sncRNA sequence reads from F1-HFD and F1-CTR males. Error bars indicate SD. (B) Principal component analysis based on 4,822 differentially expressed sncRNA species. x and y axes show principal components 1 (PC1) and 2 (PC2) that explain 83.9% and 5.6% of the total variance, respectively. (C) Composition of sncRNA transcriptomes. tsRNAs constitute the majority of sncRNAs in HFD and CTR groups. (Inset) Example of relative sncRNA coverage of tRNA-Gly-GCC-2–8. Error bars indicate SD. (D) Heat map displays relative expression of tsRNAs derived from different tRNAs across HFD and control probes. Thirteen tRNA fragments showed a significant difference in expression. Clustering bases on average linkage. The Pearson distance measurement method was used. (E) The increased level of CHRNA2 in the dSTR of F1 HFD and HFD-tsRNA offspring compared with CTR littermates. (F) Decreased levels of CHRNA2 in the Nac of F1-HFD and HFD-tsRNA offspring compared with their CTR littermates. (G) Higher expression of CHRNA2 in the VTA of F1-HFD male and HFD-tsRNA offspring compared with CTR. F1-CTR, n = 6; F1-HFD, n = 6; CTR-tsRNA, n = 12; HFD-tsRNA, n = 12. Data are presented as mean ± SEM (*P < 0.05).