Fig. 5.
JASSY binds to OPDA and forms a pore in proteoliposomes. (A) Recombinant JASSY protein was reconstituted into liposomes, and MST measurements were performed with OPDA and JA. A binding curve could be fitted only with OPDA and a KD value of 12.7 ± 3.6 µM was calculated. n = 3. (B) Liposome leakage assay. JASSY or TIC110 protein was added to liposomes, and fluorescence was recorded every millisecond (excitation at 494 nm and emission at 515 nm). Initial fluorescence was set to 0, and total liposome dequenching resulting from 0.5% Triton X-100 was set to 100%. 1× PBS buffer, pH 7.4 was added to the liposomes as a negative control.