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. 2019 Apr 29;116(21):10463–10472. doi: 10.1073/pnas.1820556116

Fig. 4.

Fig. 4.

Ancient miRNA analysis in SK29, which harbors an osteosarcoma in the pelvis. (A) Hierarchical cluster analysis of differentially expressed miRNAs (y axis) between contemporary controls and SK29 PDB-OS (x-axis). Z-score refers to high (red) and low (blue) miRNA expression using normalized values compared with the mean of total sequencing reads. (B) Box plot of controls vs. SK29 PDB-OS shows that miR-144 is highly expressed in SK29 PDB-OS. (C) Box plot of controls vs. SK29 PDB-OS shows that miR-335 is down-regulated in SK29 PDB-OS. (D) Box plot of controls vs. SK29 PDB-OS shows that miR-374 is highly expressed in SK29 PDB-OS. (E) Box plot of controls vs. SK29 PDB-OS shows that miR-451 is highly expressed in SK29 PDB-OS. (F) Digital PCR for miR-16 expression performed with pooled cDNA obtained from contemporary controls, cDNA obtained from a PDB lesion in SK29, and cDNA obtained from PDB-OS in SK29. Data are reported as copies per microliter as calculated by Poisson distribution. Error bars represent SD. Consistent with previous data, miR-16 is highly expressed in PDB-OS compared with nontransformed PDB.