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. 2019 May 8;116(21):10525–10530. doi: 10.1073/pnas.1903316116

Fig. 3.

Fig. 3.

Mice expressing MRGPRX4 scratch more after injection with unconjugated and conjugated BAs. (A) Scratching bouts associated with injection of DCA. A total of 50 µL of 1 mM DCA was injected into the nape of Ctrl and +X4 mice. Ctrl n = 13; +X4 n = 6. (B) Scratching bouts associated with injection of TDCA. A total of 50 µL of 1 mM TDCA was injected into the nape of Ctrl and +X4 mice. Ctrl n = 9; +X4 n = 5. (C) Scratching bouts associated with injection of UDCA. A total of 50 µL of 2 mM UDCA was injected into the nape of Ctrl and +X4 mice. Ctrl n = 6; +X4 n = 6. (D) Scratching bouts associated with injection of CDCA. A total of 50 µL of 2 mM CDCA was injected into the nape of Ctrl and +X4 mice. Ctrl n = 6; +X4 n = 6. (E) Scratching bouts associated with injection of human plasma from either normal or cholestatic patients (Chol) into Ctrl and +X4 mice. A total of 50 µL of plasma was injected into the nape of mice. For normal plasma: Ctrl n = 5; +X4 n = 7. For Chol plasma: Ctrl n = 5; +X4 n = 5. (A–D) Mean ± SEM depicted. Each open circle represents an individual mouse. (A–D) *P < 0.05; **P < 0.01; ***P < 0.001; two-tailed unpaired Student’s t test.