Bm-Sxl expression. (A) Tissue distributions of Bm-Sxl protein in male and female adults. Western blotting was performed using a monoclonal anti–Dm-Sxl antibody. Protein aliquots of 15 μg were loaded into each lane. (B) Schematic of a testicular follicle at the pupal stage containing spermatogonia (red), spermatocytes (blue), spermatids (yellow), and sperm (green). (C) Localization of Bm-Sxl at a testicular follicle in a 4-d-old pupa; green, blue, and red signals indicate mitochondria (Mit.), Bm-Sxl (Sxl), and nuclei (Nuc.), respectively. (Scale bar, 500 μm.)