Fig. 3.

Bm-Sxl mutants show male-specific sterility. (A) Design of TALEN target sites; arrowheads indicate target sites of TALENs. The filled box is the ORF. The blue boxes indicate RRM-type RNA binding domains. TALEN 1-2 targeted the sequence of Bm-Sxl-L–specific exon 2. TALEN 3-4 targeted the sequence of RRM1. (B) Nucleotide sequences around TALEN target sites; deletions of 13 bp (Sxl-L^Δ13), 9 bp (Sxl-L^Δ9), and 6 bp (Sxl-L^Δ6) were generated using TALEN 1-2. Deletion of 6 bp (Sxl^Δ6) and insertion of 3 bp (Sxl^In3) was achieved using TALEN 3-4. The red underline indicates the recognition sequence of TALENs. Lowercase letters indicate nonprotein encoding sequences; capital letters indicate protein-encoding regions. (C) Eggs laid by a +/+ female crossed with a +/+ male; almost all eggs were fertilized (pale to deep purple). (Scale bar, 1 cm.) (D) Eggs laid by a Sxl^In3/Sxl^In3 female crossed with a Sxl^In3/Sxl^In3 male; no eggs were fertilized (yellowish). (Scale bar, 1 cm.) (E) Fertility of Sxl^In3 mutants. Fertility was evaluated as the ratio of fertilized eggs to the total number of eggs (see Materials and Methods). The details of all fertility experiments are shown in the SI Appendix, Table S1B. n, number of crosses; error bars show SDs.