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. 2019 May 8;116(21):10473–10481. doi: 10.1073/pnas.1902805116

Fig. 3.

Fig. 3.

Kinetics of DC activation with SNAs. (A) Kinetics of antigen (OVA1) presentation and expression of costimulation marker (CD86) by BMDCs upon treatment with SNAs, determined by flow cytometry. Data points are the average of three measurements of gating, and curves obtained by fitting the data to Gaussian functions are to guide the eye. (B) Number of DLN cells from mice (n = 3) 16 h following immunization by s.c. injection with reagents as indicated. (C) Expression of costimulatory marker CD80 by DLN DCs collected from immunized mice above. (D–G) DCs isolated from immunized mice above were cocultured with purified OT1 CD8+ T cells for 48 h. Secretion of IL-12p70, IL-1α, IL-6, or TNF-α in the culture supernatant was determined by ELISA. (H) Presence of IFN-γ–secreting CD8+ T cells was measured by ELISpot assay [representative images (Left) and counts from three replicate measurements (Right)]. Data presented as mean ± SEM (B–H). ***P < 0.001; **P < 0.01; *P < 0.05.