Fig. 2.

C10 potentiates killing by INH and prevents the selection of INH-resistant mutants. (A) WT Mtb was grown in aerated planktonic conditions in Sauton’s medium with 5 μM or 25 μM C10 ± 0.25 μg/mL INH, and ODλ₆₀₀ was measured over 10 d. Mean ± SEM is graphed; n = 3. (B) The doubling time ± SD of cultures in A was calculated between day 0 and day 4. This time frame was chosen because the DMSO cultures were in the exponential growth phase. N/A indicates that growth was inhibited, and the calculation of doubling time did not accurately represent the data, as determined by R² value (R² <0.98). (C) After 10 d of treatment, cfu/mL were enumerated from cultures in A. Mean ± SEM values are graphed. n = 3. (D) WT Mtb was plated onto Sauton’s agar containing 0.5 μg/mL INH ± 25 μM C10. Representative pictures from three independent experiments are shown. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by one-way ANOVA with Tukey’s test. Complete statistical comparisons for all data are provided in SI Appendix, Table S1.