Table 5. Primer sets for real-time PCR analyses.
| Gene | Forward primer (5′ to 3′) | Reverse Primer (5′ to 3′) |
| U6 (MIM:180692) |
CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
| TBP (NM_003194) |
CCACTCACAGACTCTCACAAC | CTGCGGTACAATCCCAGAACT |
| VEZF1 (NM_007146) |
GGACAGCTATCACCTGAGGC | GCGATGGTAGAGATAAGGGGAA |
| MMP1 (NM_002421) |
AAAATTACACGCCAGATTTGCC | GGTGTGACATTACTCCAGAGTTG |
| MMP2 (NM_004530) |
TACAGGATCATTGGCTACACACC | GGTCACATCGCTCCAGACT |
| MMP9 (NM_004994) |
TGTACCGCTATGGTTACACTCG | GGCAGGGACAGTTGCTTCT |
| EDN1 (NM_001168319) |
AGAGTGTGTCTACTTCTGCCA | CTTCCAAGTCCATACGGAACAA |
| CITED2 (NM_006079) |
CCTAATGGGCGAGCACATACA | GGGGTAGGGGTGATGGTTGA |
| STMN1 (NM_203401) |
TCAGCCCTCGGTCAAAAGAAT | TTCTCGTGCTCTCGTTTCTCA |
| miRNA | Accession | Reverse transcription primers (5′ to 3′) | Forward primers (5′ to 3′) |
| miR-29a | MIMAT0004503 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTGAAC | GCGCGACTGATTTCTTTTGGT |
| miR-31 | MIMAT0000089 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCTAT | GCGAGGCAAGATGCTGGC |
| miR-138 | MIMAT0000430 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGGCCT | GCGAGCTGGTGTTGTGAATC |
| miR-191 | MIMAT0000440 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAGCTG | CGCAACGGAATCCCAAAAG |
| miR-193a-3p | MIMAT0000459 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTGGG | CGCGAACTGGCCTACAAAGT |
| mir-223-3p | MIMAT0000280 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGGGGT | GCGCGTGTCAGTTTGTCAAAT |
| miR-361 | MIMAT0000703 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTACCC | GCGCGTTATCAGAATCTCCAG |
| miR-503 | MIMAT0002874 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTGCAG | CGTAGCAGCGGGAACAGTT |
| miR-640 | MIMAT0003310 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGAGGC | CGCGATGATCCAGGAACCT |
Abbreviation: TBP: TATA-box binding protein; MMP1/2/9: matrix metallopeptidase 1/2/9; EDN1: endothelin 1; CITED2: Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2; STMN1: stathmin 1.