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. 2019 May 13;11(9):2852–2873. doi: 10.18632/aging.101959

Figure 3.

Figure 3

Demonstration of primer MARylation in Mmu WCE (A) and in the system reconstituted from recombinant proteins (B). (A) Mmu cell extract proteins (0.5 mg/mL) were incubated for 5 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 5 mM MgCl2 and 0.5 mM NAD+ in the absence or presence of PARG and olaparib. (B) Recombinant proteins were incubated for 10 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 5 mM MgCl2, 0.1 mM dNTPs, and 0.5 mM NAD+ (when indicated). Lanes 1 in A and B correspond to the initial primer (control).