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. 2019 May 21;14:3723–3741. doi: 10.2147/IJN.S197990

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© 2019 Ahmed et al.

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Evaluation of Mal-HSA-FITC uptake by different cell types. Fluorescence microscopy images of Mal-HSA-FITC probes (green) incubated for 3 h with (A) THP-1 macrophages, (C) HUVECs (EC), and (D) HCtSMCs (SMC). (B) Mal-HSA-FITC uptake by different cell types plotted against time. The results represent data from technical triplicates (9 images/well and timepoint) and are presented as mean and SEM. Data show significantly increased uptake of modified HSA probes by THP-1 cells compared with HUVEC (****P<0.0001) and HCtSMCs (^####P<0.0001) at 2.5 h and 3 h. None of the cell types recognized HSA-FITC (data not shown) (repeated measures two-way ANOVA (factors: cell type and time) and Tukey’s multiple comparison test).

Abbreviations: HSA, human serum albumin; GCU, green calibration unit.