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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Am J Med Sci. 2019 Mar 22;357(6):483–491. doi: 10.1016/j.amjms.2019.03.006

Figure 1. gp120 augments α-SMA expression in mouse primary lung fibroblasts.

Figure 1.

Mouse primary lung fibroblasts (PLFs) were isolated from wild-type mice, and after passages 3–7, cells were treated with or without gp120 (10ng/mL). (A) At 24 and 72 hours, cells were harvested for α-SMA gene expression analysis by quantitative PCR. (B) At 72 hours, cells were harvested for α-SMA protein expression analysis by Western immunoblot. (A) Untreated PLFs at 24 and 72 hours N = 12 and 11, and gp120-treated PLFs at 24 and 72 hours N = 12 and 10. (B) Untreated and gp120-treated PLFs N = 12. *P<0.05 increased compared to untreated cells.