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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Am J Med Sci. 2019 Mar 22;357(6):483–491. doi: 10.1016/j.amjms.2019.03.006

Figure 2. gp120 induces the myofibroblast phenotype in mouse primary lung fibroblasts.

Figure 2.

Mouse primary lung fibroblasts (PLFs) were isolated from wild-type mice, and after passages 3–7, PLFs were treated with (A) gp120 (10ng/mL), (B) TGFβ1 (5ng/mL), (C) no treatment, and (D) heat-inactivated gp120 (10ng/mL). At 96 hours, cells were fixed and stained for α-SMA and DAPI. Cells were analyzed for morphology by immunofluorescence microscopy. (E) Graph depicts quantification of myofibroblasts by cell count. Total PLFs counted for untreated group = 222, gp120-treated group = 371, and heat-inactivated gp120-treated group = 328. Green = α-SMA, blue = DAPI nuclear stain. All images were captured at 20× magnification. *P<0.05 increased compared with untreated and heat-inactivated gp120-treated groups.