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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Am J Med Sci. 2019 Mar 22;357(6):483–491. doi: 10.1016/j.amjms.2019.03.006

Figure 3. gp120 induces phosphorylation of ERK1/2 but not PI3K-Akt signaling.

Figure 3.

Mouse primary lung fibroblasts (PLFs) were isolated from wild-type mice, and after passages 2–7, cells were treated with or without gp120 (10ng/mL). Cells were harvested at 30, 60, or 120 minutes after treatment with gp120 and analyzed for (A) phosphorylated Akt, pan Akt, and GAPDH protein expression. Graph depicts phosphorylated Akt normalized to pan Akt. Upper panel shows representative blots. (B) phosphorylated mitogen-activated protein kinase 1/2 (ERK 1/2), total ERK 1/2, and GAPDH protein expression. Graph depicts phosphorylated ERK1/2 normalized to total ERK1/2. Upper panel shows representative blots. N = 6 per group. *P<0.05 increased compared to untreated cells.