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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Mass Spectrom Rev. 2018 Nov 25;38(3):265–290. doi: 10.1002/mas.21583

FIGURE 1.

FIGURE 1.

General lectin FLISA methodology for the measurement of glycoproteins with specific glycan motif. The method involves an antibody to selectively capture the target glycoprotein from biological samples and then a fluorophore-conjugated lectin is used to detect the glycan motif with the appropriate structural moiety. The antibody’s glycans are oxidized so they will not interfere with the analysis.