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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Mass Spectrom Rev. 2018 Nov 25;38(3):265–290. doi: 10.1002/mas.21583

FIGURE 5.

FIGURE 5.

Workflow for screening of changes in site-specific core-fucosylation (CF) of serum proteins in early stage HCC with different etiologies. The methods involve depletion of high abundance proteins, trypsin digestion of medium-to-low abundance proteins into peptides, iTRAQ labeling, and Lens culinaris Agglutinin (LCA) enrichment of CF peptides, followed by endoglycosidase F3 digestion before mass spectrometry analysis. 1300 CF peptides from 613 CF proteins were identified from patient sera, where 20 and 26 CF peptides were differentially expressed in alcohol (ALD)-related HCC samples compared with ALD-related cirrhosis samples and HCV-related HCC compared with HCV-related cirrhosis samples. Reprinted with permission from Ref [J Proteome Res 2015, 14, 4876–4884] Copyright 2015 American Chemical Society.