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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Mass Spectrom Rev. 2018 Nov 25;38(3):265–290. doi: 10.1002/mas.21583

TABLE 4.

Non-mass spectrometry methods for glycoprotein marker discovery.

Immunoassays (ELISA) Very sensitive and specific, but measures protein level but not sensitive to glycan structure
CE-based assays Specific for separation and profiling of glycans
Lectin-FLISA Specific for target glycoproteins and can detect changes in glycan structures
2-D gels/lectin analysis Can monitor global changes in the proteome for large number of proteins