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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Nat Rev Genet. 2018 Dec;19(12):770–788. doi: 10.1038/s41576-018-0059-1

Figure 2. Cytosine base editing.

Figure 2

(a) Cytosine deamination generates uracil, which base pairs as thymidine. R = 2′-deoxyribose in DNA, or ribose in RNA. (b) Cytosine base editing strategy by BE1, BE2, BE3, or BE4. R-loop formation exposes a region of single-stranded DNA to the cytidine deaminase domain. Target cytosines in this region are deaminated to uracil30,39. (c) Cellular response to cytosine base editing. Uracil DNA glycosylase-mediated excision of the uracil generated in genomic DNA is inhibited by BE2, BE3, and BE4. BE3 and BE4 are designed to nick the non-edited strand (containing the G of the original C•G target base pair), stimulating cellular DNA repair of that strand to replace the G with an A, completing the conversion of the original C•G base pair to a U•A or, following DNA replication or repair to a T•A base pair30,39.