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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Cytokine. 2019 Mar 8;119:1–6. doi: 10.1016/j.cyto.2019.02.015

Figure 3.

Figure 3

Subcellular localization of the fusion constructs between the indicated segment peptides of FLIL33 and mScI. A. Fluorescence microscopy, red channel, of two representative cells for each FLIL33 segment is shown; repeated in primary NHLFs from three separate donors, with at least 50 overexpressing cells analyzed for each donor, with consistent results. B, C. Fluorescent images of cells with DAPI-stained nuclei (blue) expressing the indicated IL-33 segment peptide – mScI fusion proteins (red), and the overlays of the two channels. D. The horizontal bars represent the corresponding FLIL33 N-terminal peptide sequences to scale; closed bars represent nuclear and open bars extranuclear or combined localization.