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A Sugita Titanium aneurysm clip with a 1.3 N closing force was used to crush the right RLN in all mice at the level of the 5th tracheal ring. A) The aneurysm clip (1 mm wide) was closed for 30 seconds to induce injury. Arrow indicates the RLN. B) Ultraviolet sterilized carbon powder was placed on the crush tool to mark the site of injury on the RLN for post-mortem identification, indicated by arrowhead. The right strap muscle is retracted laterally to allow visualization of the RLN.
