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. 2019 May;19(2):164–171. doi: 10.17305/bjbms.2019.3713

FIGURE 4.

FIGURE 4

Mechanism of MNX1-AS1 in proliferation and migration of gastric cancer (GC) cells. AGS and MGC-803 cells were transfected with si-MNX1-AS1 or si-NC. Western blot was performed to analyze the expression of PCNA, E-cadherin, N-cadherin, vimentin and MMP-9 in A) AGS and B) MGC-803 cells. GAPDH was used as the internal control. MNX1-AS1 knockdown decreased the expression of proteins involved in cell proliferation (PCNA), EMT (N-cadherin and vimentin), and metastasis (MMP-9), and upregulated E-cadherin expression in both AGS and MGC-803 cells. Quantitative data are expressed as mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001 vs. si-NC. MNX1-AS1: MNX1 antisense RNA 1; si-MNX1-AS1: Small interfering RNA (siRNA) targeting MNX1-AS1; si-NC: Non-targeting negative control siRNA; PCNA: Proliferating cell nuclear antigen; MMP-9: Matrix metallopeptidase 9; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; EMT: Epithelial to mesenchymal transition.