Fig. 2.

Scoring of prelamin A stained AG01972 fibroblasts after drug treatment. 2DD and AG01972 nuclei fixed with methanol:acetone and stained using prelamin A primary antibodies and FITC-conjugated secondary antibodies were scored by eye using an Olympus BX-41 fluorescence microscope with an UPlanFl ×100/1.30 objective lens (Olympus Corporation, Japan). The scoring patterns used for the analysis were negative, speckles, aggregates, weak rim and speckles, and strong rim and speckles. Scoring results for the treatments are shown as a FTI-277 (2.5 µM for 24 h), b pravastatin (1 µM for 24 h), c zoledronic acid (1 µM for 24 h), d rapamycin (10 nM for 24 h), e insulin-like growth factor 1 (50.0 ng ml⁻¹ for 24 h), fN-acetyl-l-cysteine (20 µM for 1 h), g FTI-277 and GGTI-2133 (both 2.5 µM for 24 h), h pravastatin and zoledronic acid (both 1 µM for 24 h) and i FTI-277 (2.5 µM for 24 h), pravastatin and zoledronic acid (both 1 µM for 24 h). Error bars are Standard error of the mean (SEM)