Fig. 8.

Scoring of progerin stained AG01972 fibroblasts after drug treatment. 2DD and AG01972 nuclei fixed with methanol:acetone and stained using progerin primary antibodies and FITC-conjugated secondary antibodies were scored by eye using an Olympus BX-41 fluorescence microscope with an UPlanFl ×100/1.30 objective lens (Olympus Corporation, Japan). The scoring patterns used for the analysis were rim, rim and speckles, speckles, and negative. Scoring results for the treatments are shown as a FTI-277 (2.5 µM for 24 h), b pravastatin (1 µM for 24 h), c zoledronic acid (1 µM for 24 h), d rapamycin (10 nM for 24 h), e insulin-like growth factor 1 (50.0 ng ml⁻¹ for 24 h), fN-acetyl-l-cysteine (20 µM for 1 h), g FTI-277 and GGTI-2133 (both 2.5 µM for 24 h), h pravastatin and zoledronic acid (both 1 µM for 24 h) and i FTI-277 (2.5 µM for 24 h), pravastatin and zoledronic acid (both 1 µM for 24 h). Error bars are SEM