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. 2019 May 21;12:4013–4023. doi: 10.2147/OTT.S209439

Figure 4.

Figure 4

The interaction between lncRNA GAS5 and miR-1323 affects proliferation and invasion of HCC cells. (A) Diagram of the predicted binding site and the corresponding mutant site for miR-1323 in the GAS5 sequence. (B) Effects of miR-1323 mimics on the activity of luciferase reporter genes containing wt or mut GAS5 sequence. Each luciferase activity was normalized to the value obtained from the cells transfected with NC mimics. (C–D) Relative GAS5 and miR-1323 expressions in SMMC7721 cells transfected with GAS5 siRNA were measured using qRT-PCR. (E) Relative GAS5 expression in SMMC7721 cells transfected with miR-1323 inhibitor was measured using qRT-PCR. (F) Western blot analysis of TP53INP1 expression in SMMC-7721 cells transfected with GAS5 siRNA. (G–H) Relative miR-1323 and GAS5 expressions in SMMC7721 cells co-transfected with GAS5 siRNA and miR-1323 inhibitor were measured using qRT-PCR. (I–J) CCK-8 and Transwell invasion assays were performed in SMMC-7721 cells co-transfected with GAS5 siRNA and miR-1323 inhibitor. Results were represented as mean ± S.D. (n≥3). *P<0.05, **P<0.01, ***P<0.001.