Table 2.
Primer Sequences for qRT-PCR
| Gene | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| miR-1305 | ACAGGCCGGGACAAGTGCAATA | GCTGTCAACGATACGCTACGTAACG |
| U6 | AACGCTTCACGAATTTGCGT | CTCGCTTCGGCAGCACA |
| UBE2T | ATCCCTCAACATCGCAACTGT | CAGCCTCTGGTAGATTATCAAGC |
| Nanog | CAGAAGGCCTCAGCACCTAC | ATTGTTCCAGGTCTGGTTGC |
| Sox2 | AGCTACAGCATGATGCAGGA | GGTCATGGAGTTGTACTGCA |
| Oct4 | CAGTGCCCGAAACCCACAC | GGAGACCCAGCAGCCTCAAA |
| GAPDH | ATGGAGAAGGCTGGGGCTC | AAGTTGTCATGGATGACCTTG |
miR-1305, microRNA-1305; UBE2T, ubiquitin-conjugating enzyme E2T; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.