TABLE 2.
Nonsynonymous SNPs in the ampD gene associated with carbapenem resistance in the URMC K. aerogenes isolates
| URMC strain group and no.a | SNP relative to ampD wild-type allelec | SNP effect on encoded AmpD sequence |
|---|---|---|
| CICU outbreak-associated CR-KA strains | ||
| 205,b 207, 209, 211, 212, 213, 215, 218, 219, 224, 225, 226 | 482G→A | Arg161His |
| 206, 208, 216 | 284G→T | Trp95Leu |
| Outbreak-unrelated CR-KA strains | ||
| 200 | 338T→G | Ile113Ser |
| 202 | 412C→Td | |
| 204 | 117C→T | Pro39Ser |
| 210 | 335C→T | Ser112Leu |
| 214 | 280G→A | Ala94Thr |
| 217 | 496G→C | Gly166Arg |
| 221 | 478A→G | Ile160Val |
| 501C→A | Ala168Asp | |
| 222 | 492T→G | Glu164Asp |
a
All strain numbers are prefixed by URMC. Control strains were URMC 201 and URMC 203; no sequencing reads mapped to the ampD gene in URMC 203. Isolates additionally resistant to cefepime are underlined.
b
Index patient isolate.
c
Unless noted otherwise, all SNPs produced missense mutations.
d
This nonsense mutation produced the substitution Gln138X, resulting in a premature stop codon.