TABLE 3.

Nonsynonymous genetic mutations in omp36 genes and resulting alterations in Omp36 sequence in the URMC K. aerogenes isolates

URMC strain group and no.a	Type of mutation in omp36b				Effect of mutation on Omp36 sequencec
	FS	HV region	NS SNP	MS SNP	FS	HV region	PS	Truncation	Single substitution(s)
CICU outbreak associated CR-KA strains
205,d 207	+	+		175A→G, 564T→C, 566A→G	pAsp91ThrfsX12		102X	+	Ile59Val
206, 208, 212, 213, 216, 224, 225, 226		+		175A→G, 564T→C, 566A→G		+			Ile59Val, Asp189Gly
209, 211, 215, 218, 219		+	184A→T	175A→G, 564T→C, 566A→G			62X	+	Ile59Val
Outbreak unrelated CR-KA strains
200, 203, 210, 214		+		564T→C, 566A→G, 615 T→G, 834T→C, 835A→G		+			Asp189Gly, Asp205Glu, Asn279Asp
202	+	+		564T→C, 566A→G, 615 T→G, 834T→C, 835A→G	pTrp77LysfsX2		78X	+	Trp77Lys
204	+	+		564T→C, 566A→G, 615 T→G, 834T→C, 835A→G	pSer304ProfsX21	+	324X	+	Asp189Gly, Asp205Glu, Asn279Asp
217		+		564T→C, 566A→G, 569T→A		+			Asp189Gly, Phe190Tyr
222		+		175A→G,564T→C, 566A→G, 615 T→G		+			Ile59Val, Asp189Gly, Asp205Glu
Control strain
201e		+		175A→G, 564T→C, 566A→G		+			Ile59Val, Asp189Gly

^aAll strain numbers are prefixed by URMC. Isolates additionally resistant to cefepime are underlined.

^bChanges shown are nucleotide positions relative to the wild-type sequence. FS, frameshift; HV, hypervariable region (nucleotides 680 to 724); NS, nonsense; MS, missense; SNP, single nucleotide polymorphism. Plus signs indicate the presence of the feature.

^cChanges shown are amino acid positions relative to the wild-type sequence. FS, frameshift; HV, hypervariable region (amino acids 226 to 241); PS, premature stop codon.

^dIndex patient isolate.

^eIntermediate carbapenem resistance.