TABLE 2.
Constructed strains used in this study
| Strain(s)a | Genotype | Source or reference |
|---|---|---|
| SC5314 | ERG11-1/ERG11-2 | ATCC |
| Constructed laboratory strainsa | ||
| 20E1II1B1 | ERG11Y132F::FRT/ERG11Y132F::FRT | 20 |
| SCERG11R1S1C1 | ERG11Y132H::FRT/ERG11Y132H::FRT | 43 |
| 10B1A3A | ERG11K143R::FRT/ERG11K143R::FRT | 20 |
| 2B1A51A | ERG11F145L::FRT/ERG11F145L::FRT | 20 |
| 29NA29A23A | ERG1E266D::FRT/ERG11E266D::FRT | 20 |
| SCERG11R3S3C1 | ERG11D278N::FRT/ERG11D278N::FRT | 43 |
| 21C1M1B1 | ERG11S405F::FRT/ERG11S405F::FRT | 20 |
| 20NA11A57A | ERG11G448E::FRT/ERG11G448E::FRT | 20 |
| 7A5A5A | ERG11F449V::FRT/ERG11F449V::FRT | 20 |
| 15A3A108A | ERG11G450E::FRT/ERG11G450E::FRT | 20 |
| 19A1A1C1 | ERG11G464S::FRT/ERG11G464S::FRT | 20 |
| 22B12A58A | ERG11D466E::FRT/ERG11D466E::FRT | 20 |
| 9A14A21A | ERG11Y132F,K143R::FRT/ERG11Y132F,K143R::FRT | 20 |
| 27A5A33A | ERG11Y132F,F145L::FRT/ERG11Y132F,F145L::FRT | 20 |
| 13A5A57A | ERG11D278N,G464S::FRT/ERG11D278N,G464S::FRT | 20 |
| 8A4A1A | ERG11G450E,I483V::FRT/ERG11G450E,I483V::FRT | 20 |
| SCTAC1GAD1A and -B | ADH1/adh1::PADH1-TAC1-GAL4AD-3xHA-caSAT1 | 45 |
| SCΔcdr1TAC1GAD1A and -B | ADH1/adh1::PADH1-TAC1-GAL4AD-3xHA-caSAT1 | 45 |
| SCMRR1GAD1B and -B | cdr1Δ::FRT/cdr1Δ::FRT ADH1/adh1::PADH1-MRR1-GAL4AD-3xHA-caSAT1 | 45 |
| SCΔmdr1MRR1GAD1A and -B | ADH1/adh1::PADH1-MRR1-GAL4AD-3xHA-caSAT1 mdr1Δ::FRT/mdr1Δ::FRT | This study |
| SCUPC2GAD1A and -B | ADH1/adh1::PADH1-UPC2-GAL4AD-3xHA-caSAT1 | 45 |
| SCUPC2R14A and -B | UPC2G648D::FRT/UPC2G648D::FRT | 32 |
a
All laboratory strains have SC5314 as the background.