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Combined PelAh and PslGh treatment of PAO1 biofilms improves antibiotic efficacy. PAO1 biofilms were treated as indicated followed by antibiotic treatment with (A) tobramycin, (B) polymyxin, (C) colistin, and (D) neomycin. Bacterial survival was determined by CFU quantification. Bars indicate mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Bonferroni’s post hoc tests. ***, P < 0.001; ****, P < 0.0001.
