FIG 2.

PslG_h treatment improves tobramycin penetration into the biofilm. Four-day-old biofilms grown under flow conditions were untreated (A) or treated with 100 nM PslG_h for 5 min (B) followed by application of Syto9- and Cy5-labeled tobramycin for 30 min. Biofilm structures were imaged with confocal microscopy (n > 9). The intensity of the tobramycin fluorescence signal (purple line) and bacterial population (green line) was measured across the surface of biofilm microcolonies to determine the location of tobramycin (bottom).