TABLE 1.
PAE, PA-SME, and SME of CF-301 against 14 S. aureus strains in 100% human seruma
| Strain (description)b | CF-301 MIC (μg/ml) | PAE (h)c | Effect (h) at MIC of:d |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0.5× |
0.25× |
0.1× |
0.05× |
|||||||
| PA-SME | SME | PA-SME | SME | PA-SME | SME | PA-SME | SME | |||
| NRS 123 (MRSA) | 1 | 3.3 ± 0.6 | 5.6 ± 0.6 | 6.0 ± 0 | 4.6 ± 0.6 | 5.0 ± 0 | 4.6 ± 0.6 | 2.6 ± 1.5 | 3.6 ± 1.5 | 1.0 ± 0 |
| JMI 227 (MRSA) | 0.5 | 3.0 ± 0 | 11.7 ± 0.6 | 9.3 ± 0.6 | 10.0 ± 0 | 5.0 ± 0 | 5.3 ± 0.6 | 4.3 ± 0.6 | 3.6 ± 1.5 | 2.7 ± 0.6 |
| JMI 22056 (MRSA) | 1 | 4.0 ± 0 | 5.3 ± 0.6 | 6.3 ± 0.6 | 4.6 ± 0.6 | 3.6 ± 1.15 | 4.6 ± 0.6 | 2.6 ± 0.6 | 3.0 ± 0 | 0.7 ± 0.6 |
| ATCC 43300 (MRSA) | 1 | 4.7 ± 0.6 | 10.0 ± 0 | 9.3 ± 0.6 | 9.6 ± 0.6 | 7.0 ± 0 | 8.6 ± 0.6 | 3.6 ± 0.6 | 7.0 ± 0 | 2.0 ± 0 |
| NRS 671 (MRSA) | 0.5 | 6.0 ± 0 | 9.6 ± 1.15 | 7.6 ± 0.6 | 7.6 ± 0.6 | 6.0 ± 0 | 5.0 ± 1 | 3.3 ± 0.6 | 4.6 ± 0.6 | 1.0 ± 0 |
| CAIRD 426 (MRSA) | 0.5 | 3.7 ± 0.6 | 5.3 ± 0.6 | 6.0 ± 0 | 5.0 ± 0 | 4.6 ± 0.6 | 4.0 ± 0 | 3.6 ± 1.5 | 3.0 ± 0 | 0 |
| ATCC 25923 (MSSA) | 1 | 5.7 ± 0.6 | 9.0 ± 0 | 12.0 ± 0 | 8.6 ± 0.6 | 10.3 ± 0.6 | 8.3 ± 0.6 | 4.6 ± 0.6 | 8.0 ± 0 | 2.3 ± 0.6 |
| ATCC 29213 (MSSA) | 1 | 8.7 ± 0.6 | 12.0 ± 0 | 12.0 ± 0 | 11.6 ± 0.6 | 5.6 ± 0.6 | 10.6 ± 0.6 | 3.0 ± 0 | 8.3 ± 0.6 | 1.6 ± 0.6 |
| NRS 121 (LRSA) | 0.5 | 4.0 ± 0 | 11.7 ± 0.6 | 12.3 ± 0.6 | 7.6 ± 0.6 | 5.3 ± 0.6 | 7.0 ± 0 | 3.6 ± 1.5 | 6.0 ± 0 | 1.0 ± 0 |
| NRS 271 (LRSA) | 0.5 | 4.0 ± 0 | 9.3 ± 0.6 | 12.0 ± 0 | 7.3 ± 0.6 | 7.3 ± 0.6 | 5.0 ± 1 | 4.6 ± 0.6 | 3.0 ± 0 | 0.7 ± 0.6 |
| AB 2145 (DRSA) | 1 | 5.3 ± 0.6 | 12.0 ± 0 | 11.6 ± 0.6 | 9.0 ± 0 | 8.3 ± 0.7 | 6.0 ± 0 | 5.0 ± 0 | 5.6 ± 0.6 | 0.7 ± 0.6 |
| CFS 954 (DRSA) | 2 | 3.6 ± 0.6 | 8.3 ± 0.6 | 11.6 ± 0.6 | 7.6 ± 0.6 | 11.0 ± 0 | 6.6 ± 1.15 | 4.0 ± 0 | 4.3 ± 0.6 | 3.3 ± 0.6 |
| VRS1 (VRSA) | 0.5 | 7.6 ± 1.15 | 9.0 ± 0 | 9.0 ± 0 | 9.0 ± 0 | 6.3 ± 1.5 | 9.3 ± 0.6 | 3.6 ± 0.6 | 9.3 ± 0.6 | 0 |
| VRS2 (VRSA) | 0.25 | 4.6 ± 1.15 | 11.7 ± 0.6 | 11.6 ± 0.6 | 8.6 ± 0.6 | 10.3 ± 0.6 | 6.0 ± 0 | 4.6 ± 0.6 | 6.3 ± 0.6 | 1.6 ± 0.6 |
| Mean | 4.8 ± 1.7 | 9.3 ± 2.4 | 9.8 ± 2.4 | 7.9 ± 2.0 | 6.8 ± 2.3 | 6.5 ± 2.0 | 3.8 ± 1.0 | 5.4 ± 2.2 | 1.3 ± 1.0 | |
a
Mean values of 3 experiments are indicated (± standard deviations).
b
MRSA, methicillin-resistant S. aureus; MSSA, methicillin-sensitive S. aureus; LRSA, linezolid-resistant S. aureus; DRSA, daptomycin-resistant S. aureus; VRSA, vancomycin-resistant S. aureus.
c
To induce the PAE, all strains were exposed to 4× MIC of CF-301 except LRSA, which was exposed to 2× MIC.
d
CF-301 concentrations (MIC values) inducing the SME in both the PA-SME and SME experimental formats are indicated.