FIG 3.

Plasmid structures determined from long-read sequencing of four isolates and inferred intermediate bla_KPC plasmid structures. (a to d) Sequenced isolates. (e to j) Inferred intermediate plasmid structures. Note that the ordering of deletion, homologous recombination, transposition, and plasmid loss events is arbitrarily represented, as the actual order of events is unknown. (k) Examples of crossover events leading to the generation of new combinations of SNVs within Tn4401 (top) or the complete swapping of Tn4401 variants between different plasmids (bottom). Black boxes indicate products of homologous recombination that were observed in long-read data (top) or Illumina data (bottom).