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. 2019 Mar 21;10:100158. doi: 10.1016/j.ynstr.2019.100158

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© 2019 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 5 — PLC activation stimulates endocannabinoid release. A. Running averages of mEPSC frequency with or without intracellular activation of PLC. With a normal patch solution, the CB1 receptor antagonist AM251 had little effect on the mEPSC frequency. With intracellular application of the PLC activator m3M3FBS via the patch pipette, AM251 caused an increase in mEPSC frequency. B. Summary bar graph of the changes in the mean normalized mEPSC frequency in response to CB1 blockade with AM251 with (m3M3FBS + AM251) and without (AM251) intracellular application of the PLC activator. *, p < 0.05.