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. 2019 May 23;21(7):689–701. doi: 10.1016/j.neo.2019.04.002

Figure 2.

Figure 2

STELA analysis of telomere length distributions in neuroblastoma cell lines. A. A schematic diagram for STELA analysis was displayed. The telorette linker is annealed to telomere G-strand overhang and ligated to the 5′ end of the C-strand. The ligated DNA is then amplified by PCR (using primers that correspond to the linker sequence and a subtelomeric sequence), and detected by Southern. B-C. STELA analysis of five different NB cell lines and HeLa. To ensure adequate coverage of the telomere size distribution, 3 to 5 parallel PCR reactions for each ligated DNA sample were performed in all STELA assays in this paper. To confirm specificity of the PCR reactions, two different probes (subtelomere-specific XpYp and telomere-specific TR82) were used. Arrowheads in B indicate several very short telomeres. Arrowheads in C highlight a few STELA fragments of very different intensity in the same reaction. Total STELA signals from each cell line analyzed in C were quantified and plotted below the blot image.