Skip to main content
. 2019 Apr 13;10:100165. doi: 10.1016/j.ynstr.2019.100165

Fig. 6.

Fig. 6

Stubby Spine Densities. Stubby spines were counted in 40 μm segments midway down the basal dendrites and at the distal termination, and in 50 μm segments midway and at the distal termination for apical dendrites and averaged to create a final spines/μm density for adolescent and adult males and females. (* = p < 0.05, post-hoc 3-way interaction; @ = p < 0.05, post-hoc 2-way interaction; ‡ = p < 0.05, age effect; # = p < 0.05, sex effect; † = p < 0.05, stress effect). A. Layer II/III.1. Basal Dendrites. Stress decreased spine density in female adults, and these stressed female adults had lower spine density than female stressed adolescents and male adult stressed rats. Group sizes were: n = 9 cells/4 rats for male adult controls, 10 cells/4 rats for stressed male adults, 9 cells/4 rats for male adolescent controls, 10 cells/4 rats for stressed male adolescents, 7 cells/4 rats for female adult controls, 14 cells/4 rats for stressed female adults, 11 cells/4 rats for female adolescent controls, 10 cells/4 rats for stressed female adolescents.

2. Apical Dendrites. There was no effect on stubby spine density. Group sizes were: n = 9 cells/4 rats for male adult controls, 10 cells/4 rats for stressed male adults, 9 cells/4 rats for male adolescent controls, 10 cells/4 rats for stressed male adolescents, 7 cells/4 rats for female adult controls, 14 cells/4 rats for stressed female adults, 11 cells/4 rats for female adolescent controls, 10 cells/4 rats for stressed female adolescents. B. Layer V.1. Basal Dendrites. Overall, males have greater spine density than females regardless of age or stress. Stress decreases spine density regardless of sex or age. Group sizes were: n = 10 cells/4 rats for male adult controls, 9 cells/4 rats for male adult defeats, 11 cells/4 rats for male adolescent controls, 10 cells/4 rats for stressed male adolescents, 9 cells/4 rats for female adult controls, 10 cells/4 rats for stressed female adults, 10 cells/4 rats for female adolescent controls, 11 cells/4 rats for stressed female adolescents. 2. Apical Dendrites. Stress decreased spine density overall and this was driven by significant effects of stress in adult males and females but not in adolescents. Stress decreases spine density in adults. Group sizes were: n = 10 cells/4 rats for male adult controls, 9 cells/4 rats for stressed male adults, 11 cells/4 rats for male adolescent controls, 10 cells/4 rats for stressed male adolescents, 9 cells/4 rats for female adult controls, 10 cells/4 rats for stressed female adults, 10 cells/4 rats for female adolescent controls, 11 cells/4 rats for stressed female adolescents.