Fig. 4.

Diazepam and vehicle oral gavage, 1 h after stress, both prevent the delayed increase in BLA spinogenesis. (a) Experimental design. Animals underwent a single 2 h episode of immobilisation stress (Stress) or remained unstressed (Control), and received either a single dose of diazepam or vehicle gavage 1 h after stress. Dendritic spine density in the BLA was quantified 10 days later. (b) Diazepam and vehicle both prevent spinogenesis in the BLA. (Control + Vehicle: n = 32 dendrites, N = 4 animals, Control + Diazepam: n = 28 dendrites, N = 4 animals, Acute Stress + Vehicle: n = 27 dendrites, N = 4 animals, Acute Stress + Diazepam: n = 29 dendrites, N = 4 animals). (c) Representative apical dendritic segments from BLA pyramidal neurons from all four groups. Dotted lines indicate mean spine density of control (no gavage) (black) and stressed (no gavage) (red) groups. ** indicates p < 0.01 and * indicates p < 0.05 in post-hoc Tukey's test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)