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. 2019 Apr 10;10:100164. doi: 10.1016/j.ynstr.2019.100164

Fig. 6.

Fig. 6

A-D. Confocal fluorescence photomicrographs showing immunolabeling of fatty acid hydrolase (FAAH), CB1r and DβH in the frontal cortex. FAAH was detected using a FITC donkey anti-rabbit IgG (green); CB1r was detected using a TRITC-conjugated donkey anti-guinea pig IgG (red) and DβH was detected using a Cy5-conjugated donkey anti-mouse IgG (blue). CB1r and DβH appeared as punctate processes. The merged image (panel D) shows co-localization of CB1 and DβH in the same process (arrows) that is in close proximity with a FAAH-labeled profile (double arrowhead). E-H. Confocal fluorescence photomicrographs showing immunolabeling of FAAH, CB1r and norepinephrine transporter (NET) in the frontal cortex. FAAH was detected using a FITC donkey anti-rabbit IgG (green); CB1r was detected using a TRITC-conjugated donkey anti-guinea pig IgG (red) and NET was detected using a Cy5-conjugated donkey anti-mouse IgG (blue). CB1r and NET appeared as punctate processes. The merged image (panel H) shows co-localization of CB1 and NET in the same process (arrows) that is in close proximity with a FAAH-labeled profile (double arrowhead). Arrowheads indicate single-labeled FAAH, CB1r or NET. Thick arrows indicate dual labeling (FAAH and DβH or CB1 and DβH). Scale bars: 30 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)