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. 2019 Apr 25;16(4):567–575. doi: 10.7150/ijms.30801

Figure 1.

Figure 1

Bone morphogenetic protein (BMP) 9 promoted osteogenic differentiation of rDFSCs. A. Fluorescence images of rat dental follicle stem/precursor cells (rDFSCs) infected with adenoviruses-expressing human BMP9 or adenoviruses-expressing green fluorescent protein (GFP). B. A quantitative ALP activity assay conducted at the indicated time points. C. Total alkaline phosphatase (ALP) staining in the wells. D. Representative images of ALP staining of rDFSCs. E. Quantitative real-time polymerase chain reaction analysis of Dlx5, OPN, Osx, and Runx2 expression. F. Alizarin Red S staining of fixed rDFSCs at 14 days post-infection. More calcified nodules were observed in the BMP9 group compared with the GFP control group. Data are representative of three independent experiments. Scale bars represent 400μm. *p<0.05, **p<0.01.