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. 2019 Apr 25;16(4):567–575. doi: 10.7150/ijms.30801

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© Ivyspring International Publisher

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.

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The effects of Matrigel on bone morphogenetic protein (BMP) 9-induced osteogenic differentiation of rat dental follicle stem/precursor cells (rDFSCs). A. Representative images of athymic nude mice injected subcutaneously with rDFSCs infected with BMP9 and mixed with Matrigel (rDFSCs-BMP9 + M) or without Matrigel (rDFSCs-BMP9). B. Representative images of tissue masses retrieved from the subcutaneous injection sites. C. Micro-computed tomography analysis and three-dimensional reconstruction of new bone formation in the two groups. D and E. Quantitative analyses of the (D) bone volume (BV) and (E) bone surface (BS) of new bones in the two groups. F. Immunohistochemical staining of tissue masses for osteo-markers. The positive images of OCN and OPN were observed in new bone tissue. Positive staining is labeled in brown. Scale bars represent 200μm.^**p<0.01.