Figure 1.

ACM increases radiosensitivity in OE33P and OE33R. OAC cells were treated with either M199 control media or ACM from non-cancer patients (n=5), ACM from surgery only OAC patients (OAC patients who had not undergone NA CRT) (n=14), or ACM from OAC patients who had previously undergone NA CRT (n=10) for 24hrs, and sensitivity to 2 Gy X-ray radiation was assessed. (A) Co-culture of OE33P cells with ACM from non-cancer patients did not significantly alter radiosensitivity. (B) Co-culture of OE33R cells with ACM from non-cancer patients significantly increased radiosensitivity, when compared to M199 control media (p=0.03). (C) OE33P cells treated with ACM from surgery only OAC patients did not demonstrate significantly altered radiosensitivity. (D) OE33R cells treated with ACM from surgery only OAC patients demonstrated significantly increased sensitivity to 2 Gy, when compared to M199 control media (p=0.002). (E) OE33P cells treated with ACM from NA CRT OAC patients demonstrated significantly increased sensitivity to 2 Gy, when compared to M199 control media (p=0.009). (F) OE33R cells treated with ACM from NA CRT OAC patients demonstrated significantly increased sensitivity to 2 Gy (p<0.0001), when compared to M199 control media. (G) OE33R cells treated with ACM from NA CRT OAC patients showed significantly increased sensitivity to 2 Gy, when compared to cells treated with ACM from surgery only OAC patients (p=0.003). Data are expressed as mean ± SEM. Statistical analysis was performed using unpaired student's t test. Statistical analysis was performed using one-way ANOVA with Tukey post-hoc correction. *p<0.05, **p<0.001, ***p<0.0001