Table 1.

Validation data of the analytical method used to determine total paclitaxel in human plasma

	Quality control samples
Parameters	LLOQ (5.00 ng/mL)	Low (15.0 ng/mL)	Medium (900 ng/mL)	High (12,000 ng/mL)
Accuracy and Precision
Intra-assay (%RE, %CV)	1.9~8.3, 3.3~6.2	−4.4~4.7, 2.6~3.9	−9.9~7.2, 1.1~2.8	−7.6~7.6, 1.3~5.7
Inter-assay (%RE, %CV)	5.8, 5.4	3.2, 3.2	−8.0, 2.7	−6.7, 2.0
Recovery (%)	/	119.0	106.2	110.1
%CV	/	2.0	2.3	1.4
Stability with accuracy
−20 °C 29 days (%RE, %CV)	/	−1.6, 4.4	/	−8.3, 2.2
−70 °C 88 days (%RE, %CV)	/	5.4, 5.5	/	0.6, 2.4
room temperature 6 hrs (%RE, %CV)	/	−3.9, 3.0	/	−8.6, 2.0
3 FT (−70 °C) (%RE, %CV)	/	6.2, 4.8	/	−4.3, 1.9
Recovery of IS (%RE, %CV)	105, 1.8

Abbreviations: LLOQ, lower limit of quantification; RE, relative error; CV, coefficient of variations; FT, freeze-thaw cycle; IS, internal standard.