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. 2019 May 21;12:4001–4011. doi: 10.2147/OTT.S195826

Figure 1.

Figure 1

The expression level of HEATR1 in NSCLC and the knockdown efficiency of HEATR1 in A549 and NCI-H460 cells. (A) The expression levels of HEATR1 in 57 pairs of NSCLC tumor and normal adjacent lung tissues were obtained from the TCGA database. HEATR1 levels were significantly up-regulated in tumor tissues (P<0.001). N=57. (B and C) The knockdown efficiency of HEATR1 was determined using qRT-PCR (B) and Western blots (C). In the mRNA level, the knockdown efficiency of shHEATR1-1 was 85.1% in A549 cells and 85.6% in NCI-H460 cells, which was the highest among three shRNAs. In protein level, HEATR1 was also significantly knocked down in both A549 and NCI-H460 cells. N=3. Values were expressed as mean ± standard deviation.