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. 2019 May 21;12:4001–4011. doi: 10.2147/OTT.S195826

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© 2019 He et al.

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The cell proliferation and cell apoptosis influenced by HEATR1 inhibition in A549 and HCI-H460 cells. (A) Celigo detection. Cell proliferation was reduced in shHEATR1 transduced cells after five days post-transduction. On 2nd, 3rd, 4th, and 5th day, the cell count was significantly smaller in shHEATR1 transduced cells compared to cells transduced with shCtrl lentivirus in both A549 and NCI-H460 cell lines (P<0.05). (B) MTT assay. HEATR1 inhibition significantly reduced cell proliferation in both A549 and NCI-H460 cells (P<0.05). (C) Colony formation assay. HEATR1 inhibition significantly decreased the colony number in A549 cells (P<0.01) and NCI-H460 cells (P<0.001). (D) Apoptosis assay. Y-axis represents whether the cells are infected with lentivirus which are carried with green fluorescence, and x-axis represents whether the cells are apoptosis which are dyed with red fluorescence. First quartile represents apoptosis cells infected with lentivirus. HEATR1 inhibition significantly increased the percentage of apoptosis cells in both A549 and NCI-H460 cells (P<0.001). N=3. Values were expressed as mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001, compared to shCtrl group.