FIGURE 2.

Knockdown of c-Abl expression inhibits LPS-induced inflammatory activation of microglia. (A) Experimental scheme. BV-2 cells were transfected with siRNA for the c-Abl gene and then treated with LPS (100 ng/ml) on day 2. Real-time PCR was performed on BV-2 cells on day 3 after transfection. (B) c-Abl gene expression was significantly reduced after siRNA transfection. (C) Nitric oxide (NO) production was measured in cells treated with LPS for 24 h after siRNA transfection. (D) TNF-α mRNA measured by real-time RT-PCR. si-Cont, control siRNA, si-Abl, siRNA for c-Abl gene knockdown. ^∗p < 0.05 vs. si-Cont from ANOVA.