FIGURE 4.

Expression of c-Abl in brain tissues after LPS injection. (A) The expression of c-Abl mRNA in brain tissues 48 h after the intraperitoneal injection of LPS was assessed by conventional RT-PCR. (B) Quantification for the c-Abl mRNA expression normalized to GAPDH. (C) Double immunostaining showed that c-Abl (red) expression co-localized with Iba-1 (green)-positive microglia in the cortex area of mouse brain 48 h post-LPS injection. Arrows indicate the double-labeled cells. The enlarged amoeboid shape of microglia is magnified as indicated in the dotted area. The nuclei were stained with DAPI (blue). ^∗p < 0.05 vs. the vehicle-treated control animals; unpaired two-tailed Student’s t test; n = 3 for each group; data are presented as mean ± SEM. Scale bar, 100 μm.