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. 2019 May 14;18:1533033819849068. doi: 10.1177/1533033819849068

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© The Author(s) 2019

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway is essential for colon cancer cells-stimulated M2 polarization of macrophage. A, Enzyme-linked immunosorbent assay (ELISA) assay showing the protein level of M2 macrophage-associated marker interleukin (IL)-6, IL-1β, Arginase-1, IL-10 in human myeloid leukemia mononuclear cells (THP-1) alone, THP-1 cocultured with HCT8 or HCT116, THP-1 treated with LY294002, and THP-1 cocultured with HCT8 or HCT116 treated with LY294002. The mean (SD) in the graph presents the relative levels from 3 replications. *P < .05, **P < .01. B, Western blot analyses showing the protein level of phosphorylated form of AKT and mTOR in THP-1 alone, THP-1 cocultured with HCT8 or HCT116, THP-1 treated with LY294002, and THP-1 cocultured with HCT8 or HCT116 treated with LY294002. GAPDH acts as internal control. C, Gray-level analysis showing the protein level of phosphorylated form of AKT and mTOR in THP-1 alone, THP-1 cocultured with HCT8 or HCT116, THP-1 treated with LY294002, and THP-1 cocultured with HCT8 or HCT116 treated with LY294002. GAPDH acts as internal control. The mean (SD) in the graph presents the relative levels from 3 replications. *P < .05, **P < .01, ***P < .001.