Skip to main content
. 2019 May 23;11:1758835919850665. doi: 10.1177/1758835919850665

Figure 6.

Figure 6.

Effects of the p53-induced miR-193a and epidermal growth factor receptor (EGFR) double-negative feedback loop on the growth of non-small cell lung carcinoma (NSCLC) xenografts in mice. (a) Representative images of tumors from mice implanted with control A549 cells, miR-193a-overexpressing A549 cells, EGFR-overexpressing A549 cells, miR-193a plus EGFR co-overexpressing A549 cells, p53-inhibiting A549 cells, or miR-193a-overexpressing plus p53-inhibiting A549 cells. A549 cells (1 × 106 cells per 0.1 ml) with different treatments were implanted subcutaneously into 6-week-old mice (3 mice per group). (b) The time course of tumor growth in the implanted mice. Tumor volume was measured every 3 days for 24 days after the inoculation. (c) Quantitative RT-PCR analysis of miR-193a levels in tumors from the implanted mice. (d) Western blotting analysis of EGFR and p53 protein levels in tumors from the implanted mice. (e) Representative images and quantitative analysis from hematoxylin and eosin (H&E), Ki67, EGFR and TUNEL staining of tumor sections obtained from the six mouse groups. All data were shown as the mean ± SEM of three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.