Table 1.
Oligonucleotide primers for real-time PCR.
| Gene | Primer sequence (5‘–3’) | NCBI Reference sequence | |
|---|---|---|---|
| ß-Actin | F | CCACACCCGCCACCAGTTCG | NM_007393.3 |
| R | TACAGCCCGGGGAGCATCGT | ||
| FLT-1/VEGFR1 | F | GCTGTTTCGGGCCCCCTTCC | NM_010228.3 |
| R | GCCTCAGAAGCCAAGCGCCA | ||
| KDR/VEGFR2 | F | GCGCGAGGTGCAGGATGGAG | NM_010612.2 |
| R | TCCCGCTGTCCCCTGCAAGT | ||
| SDF-1 | F | GCCCTTCAGATTGTTGCACGGC | NM_001012477.1 |
| R | CACTGCGGTCCATCGGCAGG | ||
| VEGFa | F | CCAGGCTGCACCCACGACAG | NM_001025250.3 |
| R | CGGACGGCAGTAGCTTCGCT | ||
| HIF-1α | F | GAAGACAACGCGGGCACCGA | NM_010431.2 |
| R | TGCTTCGCCGAGATCTTGCTGC | ||
| HIF-2α | F | GCCAAGACTGGGTTCCCGCC | NM_010137.3 |
| R | GGCTCGAACGATGGCCCCAG | ||
NCBI, National Center of Biotechnology Information; HIF, hypoxia-inducible transcription factor; SDF, stromal-cell-derived factor; VEGF, vascular endothelial growth factor.