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. 2019 Apr 18;5(5):867–873. doi: 10.1021/acscentsci.9b00147

Figure 2.

Figure 2

Ch-AOMK labels Clostridium perfringens BSH in vitro in a time- and dose-dependent manner. Active or heat-killed (HK) BSH was treated with Ch-AOMK for various amounts of time using (a) Ch-AOMK at 500 μM or (b) varying concentrations of Ch-AOMK at 37 °C for 24 h, after which the samples were tagged using the copper-catalyzed azide–alkyne cycloaddition (CuAAC) with Fluor 488-alkyne. The samples were analyzed by SDS-PAGE and visualized by in-gel fluorescence. A.U. = arbitrary unit. The bands were quantified by densitometry using ImageJ (bottom panels). Error bars represent standard deviation from the mean. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. = not significant, n = (a) 8, (b) 6.