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Metformin activates mitochondrial complex I/p-AMPK expression. (A) The activity of mitochondrial complex I was detected in cardiomyocytes. (B) The protein expression levels of p-AMPK and AMPK in cardiomyocytes were detected by western blotting. GAPDH was used as internal control. n=3. (C) The expression levels of p-AMPK and AMPK in mice were detected. n=5. *P<0.05 compared to the control group. #P<0.05 compared to the HG or the DM group.
